Publication Details (including relevant citation information):
Carbohydrate Polymers (1999), 41(4), 357-364
The glycosaminoglycans from the skin and head cartilage of the squid N. gouldi were isolated and characterized by constituent disaccharide and neutral sugar anal., 13C NMR spectroscopy, anion exchange, and size exclusion chromatog. The glycosaminoglycans from both tissues are chondroitin sulfate species. The skin consists principally of unsulfated but relatively highly glycosylated material. The chondroitin sulfate from the head cartilage is more highly sulfated, predominantly C-4,6diS (chondroitin sulfate E), with a higher mol. wt. than the skin derived material but somewhat less highly glycosylated. To provide a std. for the assignment of the 13C NMR spectrum, C-4,6diS was chem. prepd. from bovine tracheal chondroitin sulfate. This showed that it is not possible to distinguish between a mixt. of the monosulfates, C-4S and C-6S, and the C-4,6diS by 1-dimensional and simple 2-dimensional 13C NMR techniques.
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