Emiko Okamura - "Kinetics of Membrane Binding and Dissociation of 5-Fluorouracil by Pulsed Field Gradient 19F NMR"

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      Publication Details (including relevant citation   information):

      N.Yoshii and E.Okamura, Chem. Phys. Lett.,   474, 357-361 (2009).


        The kinetics of membrane binding and dissociation of an   anticancer drug, 5-fluorouracil (5FU) is quantified by high   resolution NMR with the pulsed-field-gradient technique. The   19F NMR signal of 5FU is analyzed at 293-313 K by the   solution of Bloch equation with exchange terms. The rate   constants of 5FU binding and dissociation are 0.2 and 4.1   s-1 at 303 K. The 5FU motion in the vertical direction   to the membrane surface is restricted as compared with the   lateral diffusion, judging from the activation energy (57 kJ/mol)   larger than the lateral diffusion in membrane (26 kJ/mol [E.   Okamura, N. Yoshii, J. Chem. Phys. 129  (2008) 215102]).

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