Emiko Okamura - "Real-time In-cell 19F NMR Study on Uptake of Fluorescent and Nonfluorescent 19F-Octaarginines into Human Jurkat Cells"

Document created by Emiko Okamura on Aug 22, 2014Last modified by Emiko Okamura on Apr 14, 2016
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  Publication Details (including relevant citation   information):

  E.Okamura, K.Ninomiya, S.Futaki, Y.Nagai, T.Kimura, C.Wakai,   N.Matubayasi, Y.Sugiura, and M.Nakahara, Chem. Lett.   34, 1064-1065 (2005).


  Real-time in-cell 19FNMR spectroscopy is developed   to determine the time scale of cellular uptake of   fluorescent and

  nonfluorescent 19F-octaarginines. The peptide   concentration as low as 10-mM order is   successfully detected with a

  time resolution of 1–2 min. The concentration analysis shows   that fluorescein isothiocyanate (FITC) induces different

  kinetics for the peptide uptake into natural human cells.   The ability of 19FNMR to detect the real-time uptake of   bioactive

  molecules is shown with minimal perturbation.

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