Publication Details (including relevant citation information):
E.Okamura, K.Ninomiya, S.Futaki, Y.Nagai, T.Kimura, C.Wakai, N.Matubayasi, Y.Sugiura, and M.Nakahara, Chem. Lett. 34, 1064-1065 (2005).
Real-time in-cell 19FNMR spectroscopy is developed to determine the time scale of cellular uptake of fluorescent and
nonfluorescent 19F-octaarginines. The peptide concentration as low as 10-mM order is successfully detected with a
time resolution of 1–2 min. The concentration analysis shows that fluorescein isothiocyanate (FITC) induces different
kinetics for the peptide uptake into natural human cells. The ability of 19FNMR to detect the real-time uptake of bioactive
molecules is shown with minimal perturbation.