Publication Details (including relevant citation information):
Carbohydrate Polymers (1997), 33(1), 47-56
The glycosaminoglycan (GAG) dermatan sulfate (DS) is a potentially useful drug for antithrombotic applications. Natural DS is isolated as a polymer of a mol. wt. of around 25,000. The predominant disaccharide unit is IdoA-GalNAc4SO3 but the antithrombotic activity is mediated by the binding of heparin cofactor II (HCII) to repeating units of IdoA2SO3-GalNAc4SO3. The authors hoped, in parallel with other bioactive GAGs, such as heparin, to improve the pharmacol. properties of DS by lowering the mol. wt. using oxidative free radical depolymn. The method gives a smooth and repeatable redn. in mol. wt. using chondroitin sulfate as a model system but it appears to selectively attack the disaccharide unit IdoA2SO3-GalNAc4SO3 in DS, severely reducing its activity towards HCII. In the course of these investigations the authors found that, in contrast to prior work on heparin, uncharged polysaccharides cannot be used as stds. for the detn. of mol. wt. of DS and chondroitin sulfate by high-performance size exclusion chromatog. with high ionic strength buffers.
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