Mohamed Said Attia Gaber - Novel Spectrofluorimetric Method for Measuring the Activity of the Enzyme r-L-Fucosidase Using the Nano Composite Optical Sensor Samarium(III)-Doxycycline Complex Doped in Sol-Gel Matrix

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      Publication Details (including relevant citation   information):

      Anal. Chem. 2010, 82,  6230–6236

      Abstract:

      A novel, simple, sensitive and   precise spectrofluorimetric method was developed for measuring the   activity of the enzyme α-L-fucosidase (AFU).The method was based   upon measuring the quenching of the luminescence  intensity of the produced yellow   colored complex ion associate of  2- chloro - 4-nitrophenol   (2-CNP) and a nano composite optical sensor samarium (III) – doxycycline  (Sm3+- DC) complex in sol- gel matrix at 645 nm.  The remarkable quenching of the luminescence   intensity of Sm3+- DC complex doped in sol gel matrix by various   concentrations of the reagent  2-CNP was successfully used as an optical sensor for   the assessment of AFU   activity.  The calibration plot was achieved   over the concentration range 3.4 ×10−9 - 1.0 ×10−6 mol L−1 2-CNP with a correlation coefficient of 0.999 and a   detection limit of 6.0 ×10−10 mol L−1. The method was used satisfactorily for the   assessment of the AFU activity in a number of serum samples   collected from various patients. A significant correlation between the luminescence activity   of the enzyme AFU measured by the proposed procedure and the   standard method was applied to patients and controls. The method   proceeds without practical artifacts compared to the standard   method.

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