Publication Details (including relevant citation information):
Anal. Chem. 2010, 82, 6230–6236
A novel, simple, sensitive and precise spectrofluorimetric method was developed for measuring the activity of the enzyme α-L-fucosidase (AFU).The method was based upon measuring the quenching of the luminescence intensity of the produced yellow colored complex ion associate of 2- chloro - 4-nitrophenol (2-CNP) and a nano composite optical sensor samarium (III) – doxycycline (Sm3+- DC) complex in sol- gel matrix at 645 nm. The remarkable quenching of the luminescence intensity of Sm3+- DC complex doped in sol gel matrix by various concentrations of the reagent 2-CNP was successfully used as an optical sensor for the assessment of AFU activity. The calibration plot was achieved over the concentration range 3.4 ×10−9 - 1.0 ×10−6 mol L−1 2-CNP with a correlation coefficient of 0.999 and a detection limit of 6.0 ×10−10 mol L−1. The method was used satisfactorily for the assessment of the AFU activity in a number of serum samples collected from various patients. A significant correlation between the luminescence activity of the enzyme AFU measured by the proposed procedure and the standard method was applied to patients and controls. The method proceeds without practical artifacts compared to the standard method.
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