Ying Huang - A convenient method for genetic incorporation of multiple noncanonical amino acids into one protein in Escherichia coli

Version 1

      Publication Details (including relevant citation   information):

      Mol. Biosyst. 2010, 6(4), 683-686

      Abstract:

      By overexpressing the C-terminal domain of the ribosomal protein   L11 to decrease release factor 1-mediated termination of protein   translation, enhanced amber suppression is achieved in E.   coli. This enhanced amber suppression efficiency allows the   genetic incorporation of three Ne-acetyl-L-lysines into one GFPUV   protein in E. coli.

      Address (URL): http://pubs.rsc.org/en/Content/ArticleLanding/2010/MB/b920120c