Ying Huang - Genetic incorporation of an aliphatic keto-containing amino acid into proteins for their site-specific modifications

Version 1

      Publication Details (including relevant citation   information):

      Bioorg. Med. Chem. Lett. 2010, 20(3), 878-880

      Abstract:

      2-Amino-8-oxononanoic acid has been genetically incorporated into   proteins in Escherichia coli by the use of an evolved   pyrrolysyl-tRNA synthetase/pylT pair. The direct usage of the   exclusive reactivity of the keto group in this amino acid with   hydrazide- and alkoxyamine-bearing compounds to site-specifically   label proteins under a mild condition close to physiological pH   exhibited a very high efficiency.

      Address (URL): http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TF9-4Y0T914-4&_user=9 52835&_coverDate=02/01/2010&_rdoc=19&_fmt=high&_orig=browse&_srch=doc-info%28%23 toc%235221%232010%23999799996%231609156%23FLA%23display%23Volume%29&_cdi=5221&_s ort=d&_docanchor=&_ct=119&_acct=C000049198&_version=1&_urlVersion=0&_userid=9528 35&md5=4283f901b1604f7b7be93b2d36e9e5c7