Ying Huang - Genetic incorporation of an aliphatic keto-containing amino acid into proteins for their site-specific modifications

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      Publication Details (including relevant citation   information):

      Bioorg. Med. Chem. Lett. 2010, 20(3), 878-880


      2-Amino-8-oxononanoic acid has been genetically incorporated into   proteins in Escherichia coli by the use of an evolved   pyrrolysyl-tRNA synthetase/pylT pair. The direct usage of the   exclusive reactivity of the keto group in this amino acid with   hydrazide- and alkoxyamine-bearing compounds to site-specifically   label proteins under a mild condition close to physiological pH   exhibited a very high efficiency.

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