Ying Huang - A genetically encoded photocaged N(epsilon)-methyl-l-lysine

Document created by Ying Huang on Aug 22, 2014
Version 1Show Document
  • View in full screen mode

  Publication Details (including relevant citation   information):

  Mol. Biosyst. 2010; 6(9), 1557-1560

  Abstract:

  A photocaged   Nε-methyl-L-lysine   has been genetically incorporated into proteins at amber codon   positions in Escherichia coli using an evolved   pyrrolysyl-tRNA synthetase-pylT pair. Its genetic incorporation   and following photolysis to recover   Nε-methyl-L-lysine   at physiological pH provide a convenient method for the   biosynthesis of proteins with monomethylated lysines at specific   sites.

  Address (URL): http://pubs.rsc.org/en/Content/ArticleLanding/2010/MB/C002155E

 

Attachments

    Outcomes