Ying Huang - A genetically encoded photocaged N(epsilon)-methyl-l-lysine

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  Publication Details (including relevant citation   information):

  Mol. Biosyst. 2010; 6(9), 1557-1560


  A photocaged   Nε-methyl-L-lysine   has been genetically incorporated into proteins at amber codon   positions in Escherichia coli using an evolved   pyrrolysyl-tRNA synthetase-pylT pair. Its genetic incorporation   and following photolysis to recover   Nε-methyl-L-lysine   at physiological pH provide a convenient method for the   biosynthesis of proteins with monomethylated lysines at specific   sites.

  Address (URL): http://pubs.rsc.org/en/Content/ArticleLanding/2010/MB/C002155E