Byron Brehm-Stecher - Value-added production of nisin from soy whey.

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      The objective of this study was to evaluate the potential of   low/negative value soy whey (SW) as an alternative, inexpensive   fermentation substrate to culture Lactococcus lactis subsp.   lactis for nisin production. Initially, a microtiter plate assay   using a Bioscreen C Microbiology Plate Reader was used for rapid   optimization of culture conditions. Various treatments were   examined in efforts to optimize nisin production from SW,   including different methods for SW sterilization, ultrasonication   of soy flake slurries for possible nutrient release, comparison   of diluted and undiluted SW, and supplementation of SW with   nutrients. In subsequent flask-based experiments, dry bacterial   mass and nisin yields obtained from SW were 2.18 g/L and 619   mg/L, respectively, as compared to 2.17 g/L and 672 mg/L from a   complex medium, de Man-Rogosa-Sharpe broth. Ultrasonication of   soybean flake slurries (10% solid content) in water prior to   production of SW resulted in ∼2% increase in biomass yields and   ∼1% decrease in nisin yields. Nutrient supplementation to SW   resulted in ∼3% and ∼7% increase in cell and nisin yields,   respectively. This proof-of-concept study demonstrates the   potential for use of a low/negative value liquid waste stream   from soybean processing for production of a high-value   fermentation end product.

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