Byron Brehm-Stecher - Rapid identification of Candida albicans in blood by combined capillary electrophoresis and fluorescence in situ hybridization.

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  Electrophoresis. 2010 Aug;31(16):2849-53.


  A CE method based on whole-cell molecular labeling via   fluorescence in situ hybridization was developed for the   detection of Candida albicans in whole blood. Removal of   potentially interfering red blood cells (RBC) with a simple   hypotonic/detergent lysis step enabled us to detect and   quantitate contaminating C. albicans cells at concentrations that   were orders of magnitude lower than background RBC counts (   approximately 7.0 x 10(9) RBC/mL). In the presence of the lysed   blood matrix, yeast cells aggregated without the use of a   blocking plug to stack the cells. Short (15 min) hybridizations   yielded bright Candida-specific fluorescence in situ   hybridization signals, enabling us to detect as few as a single   injected cell. The peak area response of the stacked Candida   cells showed a strong linear correlation with cell concentrations   determined by plate counts, up to approximately 10(7) CFU/mL (or   approximately 1 x 10(4) injected cells). This rapid and   quantitative method for detecting Candida in blood may have   advantageous applications in both human and veterinary   diagnostics.

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