Maria Matulova - NMR Structural study of fructans produced by Bacillus sp. 3B6, bacterium isolated in cloud water., DOI 10.1016/j.carres.2010.12.012  

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  Mária Matulová , Slavomíra Husárová , Peter Capek , Martine   Sancelme  and Anne–Marie Delort

  Carbohydr. Res. DOI   10.1016/j.carres.2010.12.012        


  Bacillus sp. 3B6,   bacterium isolated from cloud water, was incubated on sucrose for   exopolysaccharide production. Dialysis of obtained mixture (MWCO   500) afforded dialyzate (DIM) and retentate (RIM). Both were   separated by size exclusion chromatography. RIM afforded eight   fractions: levan exopolysaccharide (EPS), fructooligosaccharides   (FOSs) of levan and inulin types with different degree of   polymerization (dp 2-6) and monosaccharides fructose: glucose =   9:1. Levan was composed of two components with molecular mass ~   3500 and ~ 100kDa in the ratio 2.3:1. Disaccharide fraction   contained difructose anhydride DFA IV. 1–Kestose, 6–kestose and   neokestose were identified as trisaccharides in the ratio 2:1:3.   Fractions with dp 4-7 were mixtures of FOSs of levan   (2,6–βFruf) and   inulin (1,2–βFruf)   type. DIM separation afforded two dominant fractions:   monosaccharides with fructose: glucose ratio 1:3; disaccharide   fraction contained sucrose only.  DIM trisaccharide fraction   contained 1–kestose, 6–kestose and neokestose in the   ratio1.5:1:2, penta and hexasaccharide fractions contained FOSs   of levan type (2,6-βFruf) containing α-glucose. In   the pentasaccharide fraction also the presence of a   homopentasaccharide composed of 2,6-linked βFruf units only was identified.   Nystose, inulin (1,2–βFruf) type, was identified as DIM   tetrasaccharide. Identification of levan 2,6–βFruf and inulin   1,2–βFruf type   oligosaccharides in the incubation medium suggests both   levansucrase and inulosucrase enzymes activity in Bacillus sp. 3B6.

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