Leena Rao - Electric field effects on bioluminescent cells of Lampito mauritii: electrofusion and enhancement of bioluminescence intensity

Document created by Leena Rao on Aug 22, 2014
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  Publication Details (including relevant citation   information):

  Bioelectrochemistry and Bioenergetics 43 (1997) 197-204.

  Abstract:

  The bioluminescence of coelomic cells of Lampito mauritii has   been monitored in oxygenated phosphate buffer saline (pH 7.4)   after subjecting the coelomic cells to d.c. electric fields of   different strengths; these cells have also been examined by   electrochemical and optical techniques. The results suggest that   the electric field produces poration of the bioluminescent cells,   resulting in higher consumption of dissolved oxygen in the   medium. Enhancement of bioluminescence intensity has also    been observed. At field strengths of greater than 125 V cm-1 ,   optical microscopic examination reveals that agglomeration and   fusion of the cells has occurred.

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