Tamilselvam Batcha - Francisella targets cholesterol-rich host cell membrane domains for entry into macrophages

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      Publication Details (including relevant citation   information):

      J Immunol.  2008 Jun 15;180(12):8262-71.

      Abstract:

      Francisella tularensis is a pathogen optimally adapted to   efficiently invade its respective host cell and to proliferate   intracellularly. We investigated the role of host cell membrane   microdomains in the entry of F. tularensis subspecies holarctica   vaccine strain (F. tularensis live vaccine strain) into murine   macrophages. F. tularensis live vaccine strain recruits   cholesterol-rich lipid domains ("lipid rafts") with caveolin-1   for successful entry into macrophages. Interference with lipid   rafts through the depletion of plasma membrane cholesterol,   through induction of raft internalization with choleratoxin, or   through removal of raft-associated GPI-anchored proteins by   treatment with phosphatidylinositol phospholipase C significantly   inhibited entry of Francisella and its intracellular   proliferation. Lipid raft-associated components such as   cholesterol and caveolin-1 were incorporated into   Francisella-containing vesicles during entry and the initial   phase of intracellular trafficking inside the host cell. These   findings demonstrate that Francisella requires cholesterol-rich   membrane domains for entry into and proliferation inside   macrophages.

      Address (URL): http://www.ncbi.nlm.nih.gov/pubmed/18523292