Kelly Stewart - Deconvolution of the Cellular Oxidative Stress Response with Organelle-Specific Peptide Conjugates

Document created by Kelly Stewart on Aug 22, 2014
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  Publication Details (including relevant citation   information):

  K.P.Mahon and   T.B. Potocky, D. Blair, M.D. Roy, K.M. Stewart, T.C.   Chiles,and S.O. Kelley, Chem.Biol. 2007  14, 923.


  Oxidative stress is a deleterious force that must be combated   relentlessly by aerobic organisms and is known to underlie many   human diseases including atherosclerosis, Parkinson's disease,   and Alzheimer's disease. Information available about the   oxidative stress response has come primarily from studies using   reactive oxygen species (ROS) with ill-defined locations within   the cell. Thus, existing models do not account for possible   differences between stress originating within particular regions   of the cell. Here, oxidative stress is studied at the subcellular   level using ROS-generating compounds localizing within two   different organelles: the nucleus and the mitochondrion.   Differences in cytotoxicity, gene expression, and survival   pathway activation are detected as a function of the subcellular   origin of oxidative stress, indicating that independent   mechanisms are used to cope with oxidative stress arising in   different cellular compartments. These comparative studies,   enabled by the development of organelle-specific oxidants,   examine the cellular responses to site-specific oxidative stress   with heightened   precision.

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