Darby Kozak - Protein resistance of dextran and dextran-poly(ethylene glycol) copolymer films

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      Publication Details (including relevant citation   information):

      BIOFOULING Volume: 27  Issue: 5 Pages:  497-503 DOI:10.1080/08927014.2011.584618

      Abstract:

      The protein resistance of dextran and dextran-poly(ethylene   glycol) (PEG) copolymer films was examined on an organosilica   particle-based assay support. Comb-branched dextran-PEG copolymer   films were synthesized in a two step process using the   organosilica particle as a solid synthetic support. Particles   modified with increasing amounts (0.1-1.2 mg m(-2)) of three   molecular weights (10,000, 66,900, 400,000 g mol(-1)) of dextran   were found to form relatively poor protein-resistant films   compared to dextran-PEG copolymers and previously studied PEG   films. The efficacy of the antifouling polymer films was found to   be dependent on the grafted amount and its composition, with PEG   layers being the most efficient, followed by dextran-PEG   copolymers, and dextran alone being the least efficient.   Immunoglobulin gamma (IgG) adsorption decreased from similar to 5   to 0.5 mg m(-2) with increasing amounts of grafted dextran, but   bovine serum albumin (BSA) adsorption increased above monolayer   coverage (similar to 2 mg m(-2)) indicating ternary adsorption of   the smaller protein within the dextran layer.

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