Sandeep Kumar Vashist - Sulfo-N-hydroxysuccinimide interferes with bicinchoninic acid protein assay

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  Publication Details (including relevant citation   information):

    ANALYTICAL BIOCHEMISTRY,   417(1), 156-8,   2011


  This study revealed a major interference from   sulfo-N-hydroxysuccinimide (sulfo-NHS) in the   bicinchoninic acid (BCA) protein assay. Sulfo-NHS, a common   reagent used in bioconjugation and analytical biochemistry,   exhibited absorbance signals and absorbance peaks at 562 nm,   comparable to bovine serum albumin (BSA). However, the combined   absorbance of sulfo-NHS and BSA was not strictly additive. The   sulfo-NHS interference was suggested to be caused by the   reduction of Cu2+ in the BCA Kit’s reagent B (4%   cupric sulfate) in a manner similar to that of the protein.

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