Publication Details (including relevant citation information):
Arun, A. (2011) Comparative studies on lignin and polycyclic aromatic hydrocarbons degradation by basidiomycetes fungi. Bioresource Technology
Bajwa, Paramjit Kaur (2009) Comparative production of ligninolytic enzymes by Phanerochaete chrysosporium and Polyporus sanguineus. Canadian Journal of Microbiology 55(12)
Chaudhary, Priyanka (2011) Impact of PAH on biological health parameters of soils of an Indian refinery and adjoining agricultural area—a case study. Environmental Monitoring and Assessment
Zhang, Yinping (2011) Extracellular polymeric substances enhanced mass transfer of polycyclic aromatic hydrocarbons in the two-liquid-phase system for biodegradation. Applied Microbiology and Biotechnology
Gramss, Gerhard (2010) Mutual influence of soil basidiomycetes and white mustard plants on their enzymatic and catabolic activities. Journal of Basic Microbiology
The polycyclic aromatic hydrocarbons (PAHs) biodegradation potential of the five basidiomycetes’ fungal monocultures and their cocultures was compared with that of a Pseudomonas isolate recovered from oil-spilled soil. As utilization of hydrocarbons by the microorganisms is associated with biosurfactant production, the level of biosurfactant production and its composition by the selected microorganisms was also investigated. The Pseudomonas isolate showed higher ability to degrade three of the five PAHs but the isolate did not produce biosurfactant higher than C. versicolor and P. ostreatus. Among the PAHs, the most effective biodegradation of PAH—pyrene (42%)—was obtained with the fungus C. versicolor. Cocultures involving the fungi and Pseudomonas could not significantly degrade the selected PAHs compounds above that degraded by the most efficient monoculture. A slight increase in pyrene degradation was observed in cocultures of C. versicolor and F. palustris (93.7% pyrene). The crude biosurfactant was biochemically characterized as a multicomponent surfactant consisting of protein and polysaccharides. The PAH biodegradation potential of the basidiomycetes fungi positively correlated with their potential to express ligninolytic enzymes such as lignin peroxidase (Lip), manganese peroxidase (Mnp), and laccase. The present study utilized in silico method such as protein–ligand docking using the FRED in Open Eye software as a tool to assess the level of ligninolytic enzymes and PAHs interactions. The in silico analysis using FRED revealed that of the five PAHs, maximum interaction occurred between pyrene and all the three ligninolytic enzymes. The results of the in silico analysis corroborated with our experimental results showing that pyrene was degraded to the maximum extent by species such as C. versicolor and P. ostreatus.
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