Kamaraj Sathish kumar - Polycyclic Aromatic Hydrocarbons (PAHs) Biodegradation by Basidiomycetes Fungi, Pseudomonas Isolate, and Their Cocultures: Comparative In Vivo and In Silico Approach

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      Publication Details (including relevant citation   information):

      Arun, A. (2011) Comparative studies on lignin and polycyclic   aromatic hydrocarbons degradation by basidiomycetes fungi.   Bioresource Technology

      Bajwa, Paramjit Kaur (2009) Comparative production of   ligninolytic enzymes by Phanerochaete chrysosporium and Polyporus   sanguineus. Canadian Journal of   Microbiology 55(12)

      Chaudhary, Priyanka (2011) Impact of PAH on biological health   parameters of soils of an Indian refinery and adjoining   agricultural area—a case study. Environmental Monitoring and Assessment

      Zhang, Yinping (2011) Extracellular polymeric substances enhanced   mass transfer of polycyclic aromatic hydrocarbons in the   two-liquid-phase system for biodegradation. Applied Microbiology and Biotechnology

      Gramss, Gerhard (2010) Mutual influence of soil basidiomycetes   and white mustard plants on their enzymatic and catabolic   activities. Journal of Basic   Microbiology

      Abstract:

      Abstract

      The polycyclic aromatic hydrocarbons (PAHs) biodegradation   potential of the five basidiomycetes’ fungal monocultures and   their cocultures was compared with that of a Pseudomonas  isolate recovered from oil-spilled soil. As utilization of   hydrocarbons by the microorganisms is associated with   biosurfactant production, the level of biosurfactant production   and its composition by the selected microorganisms was also   investigated. The Pseudomonas isolate showed higher   ability to degrade three of the five PAHs but the isolate did not   produce biosurfactant higher than C. versicolor and   P. ostreatus. Among the PAHs, the most effective   biodegradation of PAH—pyrene (42%)—was obtained with the fungus   C. versicolor. Cocultures involving the fungi and   Pseudomonas could not significantly degrade the selected   PAHs compounds above that degraded by the most efficient   monoculture. A slight increase in pyrene degradation was observed   in cocultures of C. versicolor and F. palustris  (93.7% pyrene). The crude biosurfactant was biochemically   characterized as a multicomponent surfactant consisting of   protein and polysaccharides. The PAH biodegradation potential of   the basidiomycetes fungi positively correlated with their   potential to express ligninolytic enzymes such as lignin   peroxidase (Lip), manganese peroxidase (Mnp), and laccase. The   present study utilized in silico method such as protein–ligand   docking using the FRED in Open Eye software as a tool to assess   the level of ligninolytic enzymes and PAHs interactions. The in   silico analysis using FRED revealed that of the five PAHs,   maximum interaction occurred between pyrene and all the three   ligninolytic enzymes. The results of the in silico analysis   corroborated with our experimental results showing that pyrene   was degraded to the maximum extent by species such as C.   versicolor and P. ostreatus.

      Address (URL): http://www.springerlink.com/content/k151826058017340/