Mitk'El Santiago-Berrios - NADH Electrooxidation Using Bis(1,10-phenanthroline-5,6-dione)(2,2′-bipyridine)ruthenium(II)-Exchanged Zirconium Phosphate Modified Carbon Paste Electrodes

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      doi: 10.1002/elan.200503432


      We present a carbon paste electrode (CPE) modified using the   electron mediator   bis(1,10-phenanthroline-5,6-dione)(2,2′-bipyridine)ruthenium(II)   ([Ru(phend)2bpy]2+) exchanged into   the inorganic layered material zirconium phosphate (ZrP). X-Ray   powder diffraction showed that the interlayer distance of ZrP   increases upon [Ru(phend)2bpy]2+ intercalation   from 10.3 Å to 14.2 Å. The UV-vis and IR spectroscopies results   showed the characteristic peaks expected for   [Ru(phend)2bpy]2+.   The UV-vis spectrophotometric results indicate that the   [Ru(phend)2bpy]2+ concentration   inside the ZrP layers increased as a function of the loading   level. The exchanged [Ru(phend)2bpy]2+ exhibited   luminescence even at low concentration. Modified CPEs were   constructed and analyzed using cyclic voltammetry. The   intercalated mediator remained electroactive within the layers   (E°′=–38.5 mV vs.   Ag/AgCl, 3.5 M NaCl) and electrocatalysis of NADH oxidation was   observed. The kinetics of the modified CPE shows a   Michaelis–Menten behavior. This CPE was used for the oxidation of   NADH in the presence of Bakers' yeast alcohol dehydrogenase. A   calibration plot for ethanol is presented.

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