Ronald Michalsky - Concentration of the baculovirus Autographa californica M nucleopolyhedrovirus (AcMNPV) by ultrafiltration

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  Ronald Michalsky, A. Lorena   Passarelli, Peter H. Pfromm, Peter Czermak: Concentration of the   baculovirus Autographa californica M   nucleopolyhedrovirus (AcMNPV) by ultrafiltration,   Desalination 250 (2010), p. 1125-1127.


  Concentration and retention of a rod-shaped virus during   tangential flow ultrafiltration (UF) was assessed to evaluate the   potential of membrane-based downstream methods with advantages   such as easy scale-up for industrial processes. A recombinant   baculovirus of the non-spherical Autographa californica M   nucleopolyhedrovirus (AcMNPV), vHSGFP, expressing egfp was   filtered using polyethersulfone membranes ranging from 30 to 1000   kDa molecular weight cut-off (MWCO). A 20-fold virus   concentration was achieved when a membrane cut-off range of 100   to 1000 kDa was tested. Fouling was observed and cake formation   and pore plugging were postulated as concurrent causes with   different impact depending on the MWCO. A reduction of virus   concentration in the range of 2 to 5 log units in the permeate   was observed illustrating the potential of membrane-based virus   filtration as a useful unit operation in downstream processing.

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