Ronald Michalsky - Effects of temperature and shear force on infectivity of the baculovirus Autographa californica M nucleopolyhedrovirus

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      Publication Details (including relevant citation   information):

        Ronald Michalsky, Peter H. Pfromm, Peter Czermak, Christopher M.   Sorensen, A.
      Lorena Passarelli: Effects of temperature and shear force on   infectivity of the baculovirus Autographa californica M   nucleopolyhedrovirus, Journal of Virological Methods 153   (2008) 2, p. 90-96.


      Virus stability and infectivity during stressful conditions was   assessed to establish guidelines for future virus filtration   experiments and to contribute to the body of knowledge on a   widely used virus. A recombinant baculovirus of Autographa   californica M nucleopolyhedrovirus (AcMNPV), vHSGFP, was   incubated at 15–65 °C. A 2-log decrease in virus infectivity   occurred after virus incubation above 45 °C. The activation   energy of virus deactivation was circa 108 kJ/mol. Dynamic light   scattering revealed an increase in apparent virus particle size   from 150 ± 19 to 249 ± 13 nm at 55 °C. Protein and DNA   concentrations in solution correlated well with virus aggregation   as temperature was increased. Infectivity of vHSGFP stored for 5   months at 4 °C or exposed to shear stress from stirring (100 rpm,   1.02 × 10−5 psi) and pumping (50–250 ml/min, 1.45 × 10−5 to 7.25   × 10−5 psi) did not change with time. Unlike temperature   variations, cold storage and shear stress appeared to have little   impact on infectivity.

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