Publication Details (including relevant citation information):
Anal. Chem., 2011, 83 (16), pp 6121–6126
Publication Date (Web): July 15, 2011
The calcium ion response of a quartz nanopipette was enhanced by immobilization of calmodulin to the nanopore surface. Binding to the analyte is rapidly reversible in neutral buffer and requires no change in media or conditions to regenerate the receptor. The signal remained reproducible over numerous measurements. The modified nanopipette was used to measure binding affinity to calcium ions, with a Kd of 6.3 ± 0.8 × 10–5 M. This affinity is in good agreement with reported values of the solution-state protein. The behavior of such reversible nanopore-based sensors can be used to study proteins in a confined environment and may lead to new devices for continuous monitoring.
Address (URL): http://pubs.acs.org/doi/abs/10.1021/ac201322v