Boaz Vilozny - Reversible Cation Response with a Protein-Modified Nanopipette

Version 1

      Publication Details (including relevant citation   information):

      Anal. Chem., 2011, 83 (16), pp 6121–6126

      DOI: 10.1021/ac201322v

      Publication Date (Web): July 15, 2011

      Abstract:

      The calcium ion response of a quartz nanopipette was enhanced by   immobilization of calmodulin to the nanopore surface. Binding to   the analyte is rapidly reversible in neutral buffer and requires   no change in media or conditions to regenerate the receptor. The   signal remained reproducible over numerous measurements. The   modified nanopipette was used to measure binding affinity to   calcium ions, with a Kd of 6.3 ± 0.8 ×   10–5 M. This affinity is in good agreement with   reported values of the solution-state protein. The behavior of   such reversible nanopore-based sensors can be used to study   proteins in a confined environment and may lead to new devices   for continuous monitoring.

      Address (URL): http://pubs.acs.org/doi/abs/10.1021/ac201322v