Alexander KM Leung - Application of Image Analysis Techniques to Crossed Two-dimensional Immunoelectrophoresis Study

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  Computer   and Chemistry, Vol.   23(6), 577-586 (1999)


  Two-dimensional crossed immunoelectrophoresis (2D-CIEP) is a   technique widely used for studying composition of protein   mixtures in biological and clinical studies. A low cost image   analysis system with the use of an optical flatbed scanner and a   IBM-compatible PC was set up in this work for capturing 2D-CIEP   patterns. A computer package CIEPEASY was developed for   modification and analysis of the image acquired to determine   various peak parameters such as the migration distance, peak   height and peak area of the constituting components for both   qualitative and quantitative studies. In this approach, more   composition information of the standard and sample gel patterns   could be extracted from the proposed image analysis system. The   time required for data collection and interpretation of 2D-CIEP   images was shortened significantly and the results obtained have   a higher accuracy than those obtained by using conventional   methods. Moreover, a linear relationship between the peak area   and the amount of antigen present in a sample was confirmed   accurately and reported for the first time in the literature.

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