Jian Zhou - Enzyme-Based NAND and NOR Logic Gates with Modular Design

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      Publication Details (including relevant citation   information):

        J. Zhou,   M.A. Arugula, J. Halamek, M. Pita, E. Katz. Enzyme-Based NAND and   NOR Logic Gates with Modular Design. J. Phys. Chem. B,   2009, 113(49), 16065-16070.

        doi: 10.1021/jp9079052



      The logic gates NAND/NOR were mimicked by enzyme biocatalyzed   reactions activated by sucrose, maltose and phosphate. The   subunits performing AND/OR Boolean logic operations were designed   using maltose phosphorylase and cooperative work of   invertase/amyloglucosidase, respectively. Glucose produced as the   output signal from the AND/OR subunits was applied as the input   signal for the INVERTER gate composed of alcohol dehydrogenase,   glucose oxidase, microperoxidase-11, ethanol and NAD+, which   generated the final output in the form of NADH inverting the   logic signal from 0 to 1 or from 1 to 0. The final output signal   was amplified by a self-promoting biocatalytic system. In order   to fulfill the Boolean properties of associativity

      and commutativity in logic networks, the final NADH output signal   was converted to the initial signals of maltose and phosphate,   thus allowing assembling of the same standard units in   concatenated sequences. The designed modular approach, signal   amplification and conversion processes open the way toward   complex logic networks composed of standard elements resembling   electronic integrated circuitries.

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