Steven Meyers - Bioactive stent surface coating that promotes endothelialization while preventing platelet adhesion

Version 1

      Publication Details (including relevant citation   information):

      Steven R. Meyers†, Daniel J. Kenan‡, Xiaojuan Khoo†, and Mark W.   Grinstaff†

      † Departments of Biomedical Engineering and Chemistry, Metcalf   Center for Science and Engineering, 590 Commonwealth Avenue,   Boston, Massachusetts 02215, United States

      ‡ Department of Pathology, Duke University Medical Center, Box   3712, Durham, North Carolina 27710, United States

      Biomacromolecules, 2011, 12 (3), pp 533–539

      DOI: 10.1021/bm101212k

      Publication Date (Web): January 10, 2011

      Copyright © 2011 American Chemical Society

      Abstract:

      A bifunctional peptide coating was designed, synthesized, and   evaluated as a potential pro-healing stent coating. The   bifunctional peptide consisted of a short 28-mer sequence that on   the N-terminus has a motif with affinity for polystyrene binding   and at the C-terminus has a motif that was shown to bind   selectively human endothelial cells but not platelets. Results   showed that the selective coating, a polystyrene-binding peptide   terminated in RRETAWA (FFSFFFPASAWGSSGSSGK(biotin)CRRETAWAC),   bound endothelial cells quantitatively as well as the common RGD   motif, but unlike RGD, it did not show any preference for   platelet adherence. Follow-up work examining the difference in   cell line selectivity between endothelial cells, whose binding   should be encouraged, and smooth muscle cells, whose binding   should be deprecated in a stenting application, did identify a   temporal preference of the RRETAWA-terminated peptide coating for   endothelial cells. However, the in vivo implications of this   apparent selectivity need to be examined in more detail before   definitive conclusions can be drawn. The positive in vitro   results encourage the continued development of other novel   coatings that mimic biological structures, signaling   capabilities, or both to direct cellular processes on the surface   of synthetic materials.

      Address (URL): http://pubs.acs.org/doi/abs/10.1021/bm101212k