Steven Meyers - The development of peptide-based interfacial biomaterials for generating biological functionality on the surface of bioinert materials

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      Publication Details (including relevant citation   information):

      Meyers SR, Khoo X, Huang X, Walsh EB, Grinstaff MW, Kenan DJ.

      Department of Biomedical Engineering, Boston University, 44   Cummington Street, Boston, MA 02215, USA.

      Biomaterials. 2009 Jan;30(3):277-86. Epub 2008 Oct 17.

      Abstract:

      Biomaterials used in implants have traditionally been selected   based on their mechanical properties, chemical stability, and   biocompatibility. However, the durability and clinical efficacy   of implantable biomedical devices remain limited in part due to   the absence of appropriate biological interactions at the implant   interface and the lack of integration into adjacent tissues.   Herein, we describe a robust peptide-based coating technology   capable of modifying the surface of existing biomaterials and   medical devices through the non-covalent binding of modular   biofunctional peptides. These peptides contain at least one   material binding sequence and at least one biologically active   sequence and thus are termed, "Interfacial Biomaterials" (IFBMs).   IFBMs can simultaneously bind the biomaterial surface while   endowing it with desired biological functionalities at the   interface between the material and biological realms. We   demonstrate the capabilities of model IFBMs to convert native   polystyrene, a bioinert surface, into a bioactive surface that   can support a range of cell activities. We further distinguish   between simple cell attachment with insufficient integrin   interactions, which in some cases can adversely impact downstream   biology, versus biologically appropriate adhesion, cell   spreading, and cell survival mediated by IFBMs. Moreover, we show   that we can use the coating technology to create spatially   resolved patterns of fluorophores and cells on substrates and   that these patterns retain their borders in culture.

      Address (URL): http://www.sciencedirect.com/science/article/pii/S0142961208006480