Steven Meyers - Peptide-PEG amphiphiles as cytophobic coatings for mammalian and bacterial cells

Document created by Steven Meyers on Aug 22, 2014
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  Publication Details (including relevant citation   information):

  Kenan DJ, Walsh EB, Meyers SR, O'Toole GA, Carruthers EG, Lee WK,   Zauscher S, Prata CA, Grinstaff MW.

  Department of Pathology, Duke University Medical Center, Durham,   North Carolina 27710, USA.

  Chem Biol. 2006 Jul;13(7):695-700.



  Amphiphilic macromolecules containing a polystyrene-adherent   peptide domain and a cell-repellent poly(ethylene glycol) domain   were designed, synthesized, and evaluated as a cytophobic surface   coating. Such cytophobic, or cell-repellent, coatings are of   interest for varied medical and biotechnological applications.   The composition of the polystyrene binding peptide domain was   identified using an M13 phage display library. ELISA and atomic   force spectroscopy were used to evaluate the binding affinity of   the amphiphile peptide domain to polystyrene. When coated onto   polystyrene, the amphiphile reduced cell adhesion of two distinct   mammalian cell lines and pathogenic Staphylococcus aureus   strains.

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