John Owen - Platelet-activating factor.

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  Publication Details (including relevant citation   information):

  Meth Enzymol 434:105-116, 2007


  Platelet-activating factor (PAF) is a potent mediator that occurs   at very low concentrations in cells and tissues. Accurate   quantitation of PAF has always been difficult because of the   physicochemical properties of PAF and its structural similarity   to several much more abundant phospholipids. Numerous assays for   PAF have been developed, all of which have their strengths and   limitations. Herein, this chapter describes a high-pressure   liquid chromatography (HPLC)-tandem mass spectrometry assay for   PAF. Major strengths of the method are its sensitivity (detection   limit = 1 pg) and selectivity. Another advantage is that, by   using liquid instead of gas chromatography, sample derivatization   is avoided. The limitations of the method are its use of   expensive instrumentation and the requirement of performing two   HPLC runs per sample. Detailed technical advice on application of   the method to various types of samples is given.

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