Shuqin Yu - Low-Potential Detection of Endogenous and Physiological Uric Acid at Uricase−Thionine−Single-Walled Carbon Nanotube Modified Electrodes

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        This work develops and validates an electrochemical approach for   uric acid (UA) determinations in both endogenous (cell lysate)   and physiological (serum) samples. This approach is based on the   electrocatalytic reduction of enzymatically generated   H2O2    at the biosensor of uricase−thionine−single-walled carbon   nanotube/glassy carbon (UOx−Th−SWNTs/GC) with the use of Th−SWNTs   nanostructure as a mediator and an enzyme immobilization matrix.   The biosensor, which was fabricated by immobilizing UOx on the   surface of Th−SWNTs, exhibited a rapid response (ca. 2 s), a low   detection limit (0.5 ± 0.05 μM), a wide linear range (2 μM to 2   mM), high sensitivity (90   μA mM−1    cm−2),   as well as good stability and repeatability. In addition, the   common interfering species, such as ascorbic acid,   3,4-dihydroxyphenylacetic acid, 4-acetamidophenol, etc., did not   cause any interference due to the use of a low operating   potential (−400 mV vs saturated calomel electrode). Therefore,   this work has demonstrated a simple and effective sensing   platform for selective detection of UA in the physiological   levels. In particular, the developed approach could be very   important and useful to determine the relative role of endogenous   and physiological UA in various conditions such as hypertension   and cardiovascular disease.


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