Publication Details (including relevant citation information):
Methods in Molecular Biology, 857, 301-311 (2012). ISBN-10: 1617795879
Antibodies are one of the critical molecules of our immune system and are unique in their enormous diversity required for recognizing various antigens. Antibodies are protein molecules and its antigen interacting region, the fragment variable (FV) is typically composed of a light (VL) and heavy (VH) chain. In particular three loops each at the tip of the VL and the VH, known as the complementarity determining region (CDR) loops, are responsible for binding to the antigen. While the framework regions of the VL and VH are relatively constant across the entire repertoire of antibodies, the conformation of the CDR loops vary extensively to enable the antibody to recognize different antigens. Three dimensional structures of antibodies illustrating the VL-VH relative orientation and the CDR conformations are needed to gain insight into antibody stability, immunogenicity and antibody-antigen interactions. Computational modeling provides a fast and inexpensive route for generating antibody structural models. This chapter highlights the various features crucial for creating a successful antibody homology model.
Address (URL): http://dx.doi.org/10.1007/978-1-61779-588-6_13