Aroop Sircar - Modeling of Antibody Variable Regions

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  Aroop Sircar

  Methods in Molecular Biology, 857, 301-311 (2012). ISBN-10:   1617795879


  Antibodies are one of the critical molecules of our immune system   and are unique in their enormous diversity required for   recognizing various antigens. Antibodies are protein molecules   and its antigen interacting region, the fragment variable (FV) is   typically composed of a light (VL) and heavy (VH) chain. In   particular three loops each at the tip of the VL and the VH,   known as the complementarity determining region (CDR) loops, are   responsible for binding to the antigen. While the framework   regions of the VL and VH are relatively constant across the   entire repertoire of antibodies, the conformation of the CDR   loops vary extensively to enable the antibody to recognize   different antigens. Three dimensional structures of antibodies   illustrating the VL-VH relative orientation and the CDR   conformations are needed to gain insight into antibody stability,   immunogenicity and antibody-antigen interactions. Computational   modeling provides a fast and inexpensive route for generating   antibody structural models. This chapter highlights the various   features crucial for creating a successful antibody homology   model.

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