Publication Details (including relevant citation information):
Ngourn, S.; Landry, S.; Anderson, J.; Baratham, V.; Gerdon, A.E. "Identification of DNA structural motifs for hydroxyapatite binding and biomineralization" Abstracts of Papers, 240th National Meeting of the American Chemical Society, Boston, MA, 2010; INOR Poster.
Hydroxyapatite (Ca5(PO4)3OH; HAP) is the principle inorganic component in bone and enamel and the process of its formation is of current research interest. However, it is known that bioorganic molecules adhere to HAP and thereby guide its formation. DNA is a bioorganic molecule not often in contact with HAP in vivo, though is known to adhere to HAP in vitro. We have explored the binding of various DNA structural motifs to HAP using a Quartz Crystal Microbalance (QCM) biosensor, where HAP was deposited on the surface and DNA binding detected. Design of motifs focused on DNA length and level of self-hybridization (primary versus secondary structure, such as hairpin formation). We have determined equilibrium dissociation constants (Kd) ranging from 3 x 10-6 to 6 x 10-7 M, indicating a higher affinity at lower levels of hybridization. Correlation of this affinity with DNA ability to promote
biomineralization or HAP formation has also begun.
Address (URL): https://sites.google.com/site/gerdonresearchgroup/