Sunaro Ngourn - Identification of DNA structural motifs for hydroxyapatite binding and biomineralization

Version 1

      Publication Details (including relevant citation   information):

      Ngourn, S.; Landry, S.; Anderson, J.; Baratham, V.; Gerdon,   A.E. "Identification of DNA structural motifs for hydroxyapatite   binding and biomineralization" Abstracts of Papers,   240th National Meeting of the American Chemical Society, Boston,   MA, 2010; INOR Poster.

      Abstract:

      Hydroxyapatite (Ca5(PO4)3OH; HAP) is the principle inorganic   component in bone and enamel and the process of its formation is   of current research interest. However, it is known that   bioorganic molecules adhere to HAP and thereby guide its   formation. DNA is a bioorganic molecule not often in contact with   HAP in vivo, though is known to adhere to HAP in vitro. We have   explored the binding of various DNA structural motifs to HAP   using a Quartz Crystal Microbalance (QCM) biosensor, where HAP   was deposited on the surface and DNA binding detected. Design of   motifs focused on DNA length and level of self-hybridization   (primary versus secondary structure, such as hairpin formation).   We have determined equilibrium dissociation constants (Kd)   ranging from 3 x 10-6 to 6 x 10-7 M, indicating a higher affinity   at lower levels of hybridization. Correlation of this affinity   with DNA ability to promote

      biomineralization or HAP formation has also begun.

      Address (URL): https://sites.google.com/site/gerdonresearchgroup/