Sunaro Ngourn - Hydroxyapatite biomineralization through structural DNA templates

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      Publication Details (including relevant citation   information):

      Ngourn, S.; Butts, H.; Baratham, V.; Gerdon, A.E. "Hydroxyapatite   biomineralization through structural DNA templates" Abstracts   of Papers, 242nd National Meeting of the American Chemical   Society, Denver, CO, 2011; INOR Poster.


      Hydroxyapatite (Ca5(PO4)3OH; HAP) is a mineral of current   research interest. DNA is a bioorganic molecule not often in   contact with HAP in vivo, though is known to adhere to HAP in   vitro. Through Quartz Crystal Microbalance (QCM) studies, we have   determined dissociation constants (Kd) for DNA molecules binding   to HAP ranging from 0.6 to 3.0 μM. QCM has also been used to   elucidate the ability of these DNA molecules to promote   biomineralization of HAP from calcium chloride and sodium   phosphate precursors. Longer DNA molecules (80 bases) initiate   nucleation more rapidly, within 13 min, than shorter molecules   (10 bases), within 58 min. On surfaces lacking DNA, only   non-specific precipitation is observed. IR microscopy experiments   confirm the presence of HAP as opposed to amorphous phases. The   effects of self-hybridization on biomineralization with longer   DNA molecules are currently being explored. These results suggest   that biomineralization can be controlled through programmable DNA   templates.

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