Publication Details (including relevant citation information):
Ngourn, S.; Butts, H.; Baratham, V.; Gerdon, A.E. "Hydroxyapatite biomineralization through structural DNA templates" Abstracts of Papers, 242nd National Meeting of the American Chemical Society, Denver, CO, 2011; INOR Poster.
Hydroxyapatite (Ca5(PO4)3OH; HAP) is a mineral of current research interest. DNA is a bioorganic molecule not often in contact with HAP in vivo, though is known to adhere to HAP in vitro. Through Quartz Crystal Microbalance (QCM) studies, we have determined dissociation constants (Kd) for DNA molecules binding to HAP ranging from 0.6 to 3.0 μM. QCM has also been used to elucidate the ability of these DNA molecules to promote biomineralization of HAP from calcium chloride and sodium phosphate precursors. Longer DNA molecules (80 bases) initiate nucleation more rapidly, within 13 min, than shorter molecules (10 bases), within 58 min. On surfaces lacking DNA, only non-specific precipitation is observed. IR microscopy experiments confirm the presence of HAP as opposed to amorphous phases. The effects of self-hybridization on biomineralization with longer DNA molecules are currently being explored. These results suggest that biomineralization can be controlled through programmable DNA templates.
Address (URL): https://sites.google.com/site/gerdonresearchgroup/