Publication Details (including relevant citation information):
Vargas-Medrano, J., Sierra-Fonseca, A. J., Carrillo-Arellano, M., and Plenge-Tellechea, L. F. (2011). Cypermethrin, deltamethrin and glyphosate affect the activity of the human Ca2+-ATPase. Tecnociencia Chihuahua. 5(3):111-120.
The extensive use of pesticides can cause many human health problems. However, the effects of pesticides on a biochemical level are still poorly understood. In this study we analyzed the effect of different pesticides on the plasma membrane Ca2+-ATPase (PMCA) and on the sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) activities. Different amounts of pesticides were added to the Ca2+-dependent ATPase assays in order to determine if they were affecting the ATPase activity. The results showed that PMCA activity was partially inhibited by deltamethrin to 51.85% ± 3.7 when its concentration in the reaction medium was 0.5 mM, while cypermethrin and glyphosate stimulated the PMCA activity at the lowest concentrations tested. The maximum stimulatory effect of cypermethrin was of 155% ± 9.0 at a concentration of 0.2 mM. In addition, the herbicide glyphosate stimulated the activity 111% ± 2.0 at a concentration of 0.2 mM. In conclusion, our results showed that PMCA activity was partially inhibited by deltamethrin, but cypermethrin and glyphosate stimulated their activity. Our findings suggest that cypermethrin and deltamethrin have different structure-activity relationships. However, SERCA was not sensitive to deltamethrin or glyphosate. These differences may be reflected in disturbs over cellular calcium regulation.