John O'Neill - DNA SEQUENCING METHODS AND DETECTORS AND SYSTEMS FOR CARRYING OUT THE SAME

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      Publication Details (including relevant citation   information):

      US 2011/0236984 A1   Sep. 29, 2011

      DNA   SEQUENCING METHODS AND DETECTORS AND SYSTEMS FOR CARRYING OUT THE   SAME

      H. Sun, E.S.   Nordman, M.F. Oldham, J.R. O’Neill, C. Connell, U. Ulmanella,   A.N.K. Lau, T. Kotseroglou, and K.J. Livak

      LIFE TECHNOLOGIES   CORPORATION

      Abstract:

        In some embodiments, an analyte detection system is provided that   includes a nanochannel, an electrode arrangement, and a plurality   of nanoFET devices disposed in the nanochannel. A plurality of   nucleic acid base detection components can be used that include a   plurality of nanopores, a plurality of nanochannels, a plurality   of hybridization probes, combinations thereof, and the like.   According to other embodiments of the present teachings,   different coded molecules are hybridized to a target DNA molecule   and used to detect the presence of various sequences along the   target molecule. A kit including mixtures of coded molecules is   also provided. In some embodiments, devices including   nanochannels, nanopores, and the like, are used for manipulating   movement of DNA molecules, for example, in preparation for a DNA   sequencing detection. Nanopore structures and methods of making   the same are also provided as are methods of nucleic acid   sequencing using the nanopore structures. Surface-modified   nanopores are provided as are methods of making them. In some   embodiments, surfaced-modified nanopores for slowing the   translocation of single stranded DNA (ssDNA) through the nanopore   are provided, as are nanopores configured to detect each of a   plurality of different bases on an ssDNA strand.

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