Gilda Shayan - Synthesis and Characterization of High-Throughput Nanofabricated Poly(4-hydroxy styrene) Membranes for <i>In vitro</i> Models of Barrier Tissue.

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  Publication Details (including relevant citation   information):

  Tissue Eng Part C   Methods. 2012 Mar 21. [Epub ahead of print]


  Commercially available permeable supports with microporous   membranes have led to significant improvements in the culture of   polarized cells because they permit them to feed basolaterally   and thus carry out metabolism in a more <i>in   vivo</i> like setting. The porous nature of these membranes   enable permeability measurements of drugs or biomolecules across   the cellular barrier. However, current porous membranes have a   high flow resistance due to great thickness (20-40 μm), low   porosity, and a wide pore size distribution with tortuous   diffusion paths which make them low-throughput for permeability   studies. Here we describe an alternate platform that is more   flexible, allows for more control over physical parameters of the   membranes, and is high-throughput. This study reports on the   synthesis, nanofabrication, and surface characterization of a 3   μm thick, transparent membrane based on poly(4-hydroxy styrene).   The membranes are nanofabricated using electron beam lithography   and deep ion plasma etching to achieve an organized array of   straight pores from 50 nm to 800 nm in diameter, with at least 23   times less flow resistance. It also shows for the first time the   potential utility of poly(4-hydroxy styrene) as a cell culture   substrate with lack of cytotoxicity, and suitability for   nanofabrication processes due to temperature stability.

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