Gilda Shayan - The effect of astrocytes on the induction of barrier properties in aortic endothelial cells.

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  Biotechnol Prog. 2011 Apr 20;   27(4): 1137-1145. Epub 2011 May 27.


  Construction of in vitro models of the blood-brain barrier (BBB)   using primary brain microvascular endothelial cells (BMEC) is   time intensive and not high throughput, in part due to a lack of   culture purity, low yields, and cellular dedifferentiation after   the first passage. This problem has created interest in the   substitution of BMEC with immortalized brain endothelial cells   (EC), or peripheral EC such as bovine aortic EC (BAEC). Many BBB   models have focused on further inducing the brain and peripheral   ECs by incorporating astrocyte back-to-back or nonback-to-back   cocultures. However, previous studies demonstrating induction   effects of astrocytes on BAEC in back-to-back cocultures failed   to recognize the extensive barrier properties of astrocytes   alone, which can have a significant effect on interpreting the   results. This manuscript reports the establishment of   back-to-back and nonback-to-back cocultures between astrocytes   and BAEC or BMEC (as a control) with primary focus on the   properties of astrocytes alone and with a linear contrast   statistical methodology to interpret the results.   Transendothelial electrical resistance and permeability studies   revealed that astrocytes can significantly increase the barrier   tightening of BMEC by 167%, while having no effect on BAEC.   Immunocytochemical studies also revealed the reorganization of   BMEC occludin junctions in the presence of astrocytes, while   indicating the absence of this junctional protein in BAEC. In   contrast to a previous report, here the linear contrast   statistical analysis revealed that observed decreases in   permeability of BAEC in back-to-back cocultures is due to the   addition of astrocytes' properties in series and not due to   induction. © 2011 American Institute of Chemical Engineers   Biotechnol. Prog., 2011.

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