Karen Morrison - Whole cell microtubule analysis by flow cytometry

Version 1

      Publication Details (including relevant citation   information):

      Analytical Biochemistry, Volume 420, Issue 1, 1 January 2012,   Pages 26–32


      Perturbation of the tubulin/microtubule dynamic in cells is   perhaps the single most important mode of action of anticancer   drugs. Standard methods for identifying and evaluating compounds   for their ability to alter tubulin polymerization are low   throughput, labor intensive, expensive, or make their assessment   in vitro. Here we report a method to rapidly quantify the extent   of tubulin polymerization in whole cells using flow cytometry,   and we use this technique to evaluate compounds that stabilize   and destabilize microtubule formation. This facile method is   useful for conveniently, quantitatively, and cost-effectively   comparing small molecules that perturb tubulin polymerization.

      Address (URL): http://dx.doi.org/10.1016/j.ab.2011.08.020