Sunaro Ngourn - Calcium phosphate Biomineralization through structural DNA templates

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      Publication Details (including relevant citation   information):

      Ngourn, S.; Butts, H.; Petty, A.; Baratham, V.; Gerdon, A.E.   "Calcium phosphate Biomineralization through structural DNA   templates" 4th Annual Northeastern Undergraduate Research and   Development Symposium, Biddeford, ME, 2012; Poster.


      Hydroxyapatite (Ca5(PO4)3OH;   HAP) is the principle inorganic component in bone and enamel and   the process of its formation is of current research interest. It   is known that bioorganic molecules adhere to HAP and thereby   guide its formation. DNA is a bioorganic molecule not often in   contact with HAP in vivo, though is known to adhere to HAP in   vitro. Through Quartz Crystal Microbalance (QCM) studies, we have   determined dissociation constants (Kd) for DNA molecules binding   to HAP ranging from 0.6 to 3.0 micro molar. QCM has also been   used to elucidate the ability of these DNA molecules to promote   biomineralization of HAP from calcium chloride and sodium   phosphate precursors. Longer DNA molecules (80 bases) initiate   nucleation more rapidly, within 13 min, than shorter molecules   (10 bases). On surfaces lacking DNA, only non-specific   precipitation is observed. IR microscopy experiments suggest the   presence of HAP as opposed to amorphous phases. DNA in solution   also enhanced mineralization though different kinetics where   shorter strands had a more pronounced effect. The effects of   self-hybridization on biomineralization with longer DNA molecules   are currently being explored. These results suggest that DNA   templates can quickly and controllably initiate   biomineralization.

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