Elizabeth Crew - MicroRNA Conjugated Gold Nanoparticles and Cell Transfection

Document created by Elizabeth Crew on Aug 22, 2014
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  Publication Details (including relevant citation   information):

  E. Crew, S. Rahman, A.   Razzak-Jaffar, D. Mott, M. Kamundi, G. Yu, N. Tchah, J. Lee, M.   Bellavia, and C. J.Zhong, Anal. Chem.,   2012, 34, 26-29.


  While the importance of microRNAs (miRNAs) in cancer treatment or   manipulation of genetic expression has been increasingly   recognized for developing miRNA-based therapies, the controlled   delivery of miRNAs into specific cells constitutes a challenging   task. This report describes preliminary findings from an   investigation of the conjugation of gold nanoparticles with   miRNAs (miRNA−AuNPs) and their cell transfection. The   immobilization of miRNAs on the AuNPs was detected, and the   surface stability was substantiated by gel electrophoresis   assessment of the highly charged characteristics of miRNA−AuNPs   and their surface-exchange inactivity with a highly charged   surfactant. The miRNA−AuNPs were tested in cell transfection   using multiple myeloma cells, demonstrating efficient knockdown   in the functional luciferase assay. The findings have important   implications for understanding the mechanistic details of cell   transfection involving miRNA-conjugated nanoparticles as   biosensing or targeting probes.

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