Ivan Kempson - In situ analysis of metal(loid)s in plants: state of the art and artefacts

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      Publication Details (including relevant citation   information):

      E Lombi, KG   Scheckel, IM Kempson, In situ analysis   of metal(loid)s in plants: state of the art and artefacts.   Environmental and Experimental Botany, 72,3-17,   2011.


      Metals and metalloids play important roles in plant function and   metabolism. Likewise, plants subsequently introduce vital dietary   nutrition to people and animals. Understanding the transport,   localisation and speciation of these elements is critical for   understanding availability and metabolic pathways. Subsequently   this knowledge can be applied to plant physiology and   agricultural research, food science and genetic engineering. This   review focuses on the most recent status of in situ techniques to   visualise spatial distributions and assess the speciation of   metals and metalloids. The techniques addressed include:   histochemical analysis, autoradiography, LA-ICP-MS, SIMS, SEM   including EDX, PIXE; and synchrotron methods: XRF, differential

      and fluorescence tomography, and X-ray absorption techniques.   This review has been written with the intent of plant researchers   to gain familiarity with techniques to which they are not   accustom but wish to extend their research with alternative, but   complementary, capabilities. Importantly, the disadvantages as   well as advantages, have been highlighted for each technique and   potential artefacts induced by the analysis or sample preparation   are reviewed. These often overlooked aspects are the points   critical for novice use of unfamiliar techniques and are offered   for advancing research approaches commensurate with the   accelerating interest regarding metal(loid)s in botanical   specimens.

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