Nazmiye Yapici - Determination of intracellular pH using sensitive, clickable fluorescent probes

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      Publication Details (including relevant citation   information):

      N. Yapici et al, Bioorg. Med. Chem. Letts,22,   2012, 2240-2243, DOI:10.1016/j.bmcl.2012.02.021


        We synthesized and evaluated a series of acidic fluorescent pH   probes exhibiting robust pH dependence, high sensitivity and   photostability, and excellent cell membrane permeability.   Titration analyses indicated that probe   3   could increase its fluorescence intensity 800-fold between pH 8.0   and 4.1. Additionally, its pKavalue   is optimal for intracellular probing of acidic organelles.   Fluorescent imaging of HepG2 and Hela cells further revealed that   probe   3   demonstrates outstanding capacity for monitoring of intracellular   [H+]   levels. The easily accessible terminal alkyne/azido function   groups of these probes offer the possibility of rapidly   constructing sensor molecule libraries using ‘click’   chemistry.

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