Publication Details (including relevant citation information):
N. Yapici et al, Bioorg. Med. Chem. Letts,22, 2012, 2240-2243, DOI:10.1016/j.bmcl.2012.02.021
We synthesized and evaluated a series of acidic fluorescent pH probes exhibiting robust pH dependence, high sensitivity and photostability, and excellent cell membrane permeability. Titration analyses indicated that probe 3 could increase its fluorescence intensity 800-fold between pH 8.0 and 4.1. Additionally, its pKavalue is optimal for intracellular probing of acidic organelles. Fluorescent imaging of HepG2 and Hela cells further revealed that probe 3 demonstrates outstanding capacity for monitoring of intracellular [H+] levels. The easily accessible terminal alkyne/azido function groups of these probes offer the possibility of rapidly constructing sensor molecule libraries using ‘click’ chemistry.
Address (URL): http://dx.doi.org/10.1016/j.bmcl.2012.02.021