Michael Marty - Native Mass Spectrometry Characterization of Intact Nanodisc Lipoprotein Complexes

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  Publication Details (including relevant citation   information): Marty, Michael T., Zhang, Hao, Cui,   Weidong, Blankenship, Robert E., Gross, Michael L., Sligar,   Stephen G., Anal. Chem., 2012,   84 (21), pp 8957-8960

  Abstract: We describe here the analysis of   nanodisc complexes by using native mass spectrometry (MS) to   characterize their molecular weight (MW) and polydispersity.   Nanodiscs are nanoscale lipid bilayers that offer a platform for   solubilizing membrane proteins. Unlike detergent micelles,   nanodiscs are native-like lipid bilayers that are well-defined   and potentially monodisperse. Their mass spectra allow peak   assignment based on differences in the mass of a single lipid per   complex. Resultant masses agree closely with predicted values and   demonstrate conclusively the narrow dispersity of lipid molecules   in the nanodisc. Fragmentation with collisionally activated   dissociation (CAD) or electron-capture dissociation (ECD) shows   loss of a small number of lipids and eventual collapse of the   nanodisc with release of the scaffold protein. These results   provide a foundation for future studies utilizing nanodiscs as a   platform for launching membrane proteins into the gas phase.

  Address (URL): http://pubs.acs.org/doi/abs/10.1021/ac302663f