Michael Marty - Nanodiscs as a launch pad for membrane protein native mass spectrometry.

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  Publication Details (including relevant citation   information): Marty,Michael T., Zhang,Hao, Cui,Weidong,   Blankenship,Robert E., Gross,Michael L., Sligar,Stephen G.,   Abstracts of Papers, 244th ACS National Meeting &   Exposition, Philadelphia, PA, United States, August 19-23,   2012, 2012, pp ANYL-211

  Abstract: Despite their importance, structural   biol. of membrane proteins has lagged behind that of cytosolic   proteins. Emerging mass spectrometry techniques, including   footprinting and native mass spectrometry, provide information on   membrane protein structure that complement established methods.   Nanodiscs are nanoscale lipid bilayers encircled by membrane   scaffold protein belts that provide a platform for solubilizing   membrane proteins. We have utilized native mass spectrometry to   study the intact Nanodisc supramol. complex. Native mass   spectrometry measurements of Nanodiscs of different sizes and   lipid compns. yield mass distributions in line with predicted   values. Interestingly, the presence of anionic lipids does not   significantly alter the charge state, presumably due to shielding   by residual counter ions. Collision induced dissocn. releases the   incorporated target membrane protein for tandem mass   spectrometry. The ability to introduce intact Nanodiscs into a   mass spectrometer provides a unique launch pad for studying   membrane protein complexes, protein-lipid interactions, and for   top-down investigations on membrane proteins. [on SciFinder(R)]

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