Brandon Russell - A system for exposing molecules and cells to biologically relevant and accurately controlled steady-state concentrations of nitric oxide and oxygen

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      Publication Details (including relevant citation   information): Dendroulakis, Vasileios, Russell, Brandon   S., Eric Elmquist, C., Trudel, Laura J., Wogan, Gerald N., Deen,   William M., Dedon, Peter C., Nitric Oxide,   2012, 27 (3), pp 161-168

      Abstract: Nitric oxide (NO) plays key roles in   cell signaling and physiology, with diverse functions mediated by   NO concentrations varying over three orders-of-magnitude. In   spite of this critical concentration dependence, current   approaches to NO delivery in vitro result in biologically   irrelevant and poorly controlled levels, with hyperoxic   conditions imposed by ambient air. To solve these problems, we   developed a system for controlled delivery of NO and O2 over   large concentration ranges to mimic biological conditions. Here   we describe the fabrication, operation and calibration of the   delivery system. We then describe applications for delivery of NO   and O2 into cell culture media, with a comparison of experimental   results and predictions from mass transfer models that predict   the steady-state levels of various NO-derived reactive species.   We also determined that components of culture media do not affect   the steady-state levels of NO or O2 in the device. This system   provides critical control of NO delivery for in vitro models of   NO biology and chemistry.

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