Publication Details (including relevant citation information): Lewandowski, Bartosz, De Bo, Guillaume, Ward, John W., Papmeyer, Marcus, Kuschel, Sonja, Aldegunde, María J., Gramlich, Philipp M. E., Heckmann, Dominik, Goldup, Stephen M., D’Souza, Daniel M., Fernandes, Antony E., Leigh, David A., Science , 2013, 339 (6116 ), pp 189 -193
Abstract: The ribosome builds proteins by joining together amino acids in an order determined by messenger RNA. Here, we report on the design, synthesis, and operation of an artificial small-molecule machine that travels along a molecular strand, picking up amino acids that block its path, to synthesize a peptide in a sequence-specific manner. The chemical structure is based on a rotaxane, a molecular ring threaded onto a molecular axle. The ring carries a thiolate group that iteratively removes amino acids in order from the strand and transfers them to a peptide-elongation site through native chemical ligation. The synthesis is demonstrated with ~1018 molecular machines acting in parallel; this process generates milligram quantities of a peptide with a single sequence confirmed by tandem mass spectrometry.
Address (URL): http://www.sciencemag.org/content/339/6116/189.abstract