Lincoln Scott - Strong coupling effects during X-pulse CPMG experiments recorded on heteronuclear ABX spin systems: artifacts and a simple solution.

Version 1

      Publication Details (including relevant citation   information):

      J Biomol NMR.  2007 May;38(1):41-6. Epub 2007 Mar 3.

      Abstract:

      Simulation and experiment have been used to establish that   significant artifacts can be generated in X-pulse CPMG relaxation   dispersion experiments recorded on heteronuclear ABX   spin-systems, such as (13)C( i )-(13)C( j )-(1)H, where (13)C( i   ) and (13)C( j ) are strongly coupled. A qualitative explanation   of the origin of these artifacts is presented along with a simple   method to significantly reduce them. An application to the   measurement of (1)H CPMG relaxation dispersion profiles in an   HIV-2 TAR RNA molecule where all ribose sugars are protonated at   the 2' position, deuterated at all other sugar positions and   (13)C labeled at all sugar carbons is presented to illustrate the   problems that strong (13)C-(13)C coupling introduces and a simple   solution is proposed.

      Address (URL): http://www.ncbi.nlm.nih.gov/pubmed/17334825