Lincoln Scott - Enthalpy arrays.

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      Publication Details (including relevant citation   information):

        Proc Natl Acad Sci U S A. 2004 Jun 29;101(26):9517-22.   Epub 2004 Jun 21.


      We report the fabrication of enthalpy arrays and their use to   detect molecular interactions, including protein-ligand binding,   enzymatic turnover, and mitochondrial respiration. Enthalpy   arrays provide a universal assay methodology with no need for   specific assay development such as fluorescent labeling or   immobilization of reagents, which can adversely affect the   interaction. Microscale technology enables the fabrication of   96-detector enthalpy arrays on large substrates. The reduction in   scale results in large decreases in both the sample quantity and   the measurement time compared with conventional microcalorimetry.   We demonstrate the utility of the enthalpy arrays by showing   measurements for two protein-ligand binding interactions (RNase A   + cytidine 2'-monophosphate and streptavidin + biotin),   phosphorylation of glucose by hexokinase, and respiration of   mitochondria in the presence of 2,4-dinitrophenol uncoupler.

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