Lincoln Scott - Improvement in the apparent mass resolution of oligonucleotides by using 12C/14N-enriched samples.

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      Publication Details (including relevant citation   information):

      Anal Chem. 2002 Jan   1;74(1):226-31.


      The apparent mass resolution of oligonucleotides in   time-of-flight (TOF) mass spectrometers has been examined. In a   reflectron TOF instrument, where the isotopic profile can be   completely resolved, the apparent resolution matches the   instrument's resolving power. In a linear TOF instrument,   unresolved isotopic profiles limit the apparent resolution to   much lower values than the actual instrument resolution. By using   12C/14N-enriched oligonucleotides, the apparent resolution can be   improved significantly. The isotope enrichment method also   enhances the signal-to-noise ratio.

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