Lee Shekter - Molecular cloning, expression, and pharmacological characterization of humEAA1, a human kainate receptor subunit.

Document created by Lee Shekter on Aug 22, 2014
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  Publication Details (including relevant citation   information): Kamboj,R.K., Schoepp,D.D., Nutt,S.,   Shekter,L., Korczak,B., True,R.A., Rampersad,V., Zimmerman,D.M.,   Wosnick,M.A., J Neurochem, 1994,   62 (1), pp 1-9

  Abstract: Kainate is a potent neuroexcitatory   agent; its neurotoxicity is thought to be mediated by an   ionotropic receptor with a nanomolar affinity for kainate. In   this report, we describe the cloning of a cDNA encoding a human   glutamate ionotropic receptor subunit protein from a human   hippocampal library. This cDNA, termed humEAA1, is most closely   related to rat and human cDNAs for kainate receptor proteins and,   when expressed in COS or Chinese hamster ovary cells, is   associated with high-affinity kainate receptor binding. We have   successfully established cell lines stably expressing humEAA1.   This is the first report of establishment of stable cell lines   expressing a glutamate receptor subunit. The relative potency of   compounds for displacing [3H]kainate binding of humEAA1 receptors   expressed in these stable cell lines was kainate > quisqualate   > domoate > L-glutamate >   (RS)-alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid   > dihydrokainate > 6,7-dinitroquinoxaline-2,3-dione >   6-cyano-7-nitroquinoxaline-2,3-dione. Homooligomeric expression   of humEAA1 does not appear to elicit ligand-gated ion channel   activity. Nevertheless, the molecular structure and   pharmacological characterization of high-affinity kainate binding   of the humEAA1 expressed in the stable cell line (ppEAA1-16)   suggest that the humEAA1 is a subunit protein of a human kainate   receptor complex.

  Address (URL): http://www.hubmed.org/display.cgi?uids=8263508