rofiq sunaryanto - Optimization of Streptomyces sp.A11 Medium Cultivation on Cyclo(Tyrosyl-Prolyl) Production Using the Response Surface Methodology

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      International Journal of WASTE RESOURCES   (IJWR) vol.2 No.2. 2012


      The Response Surface Methodology is a three factorial design   which provides the relationship between one or more measured   dependent response and a number of input (independent) factors.   Response surface methodology was used for optimization of   fermentation medium for antibacterial agent cyclo(tyr-pro)   production by Streptomyces sp. A11. The optimal response   region of the significant factor was predicted by using a second   order polynomial model fitted to the results obtained by applying   the Central Composite Design (CCD) statistical design.   Determination of the best production medium of cyclo(tyr-pro)   showed among 6 carbon sources used, dextrin produced the highest   cyclo(tyr-pro). Among the five nitrogen sources used, peptone   produced the highest cyclo(tyr-pro), and the mineral salts group   I produced the highest cyclo(tyr-pro) compared with others. The   results of optimization showed that three variables (dextrin as a   carbon source, peptone as nitrogen source, and mixture of mineral   salts) used showed significantly affected to the activity of   cyclo(tyr-pro). The mathematical model used, obtained by the   composition of the three variables most optimum were   concentration of dextrin was 32.55 g.L-1, peptone   concentration was 11.22 g L-1, and 8.65 mL of mineral   salts. By using the composition of mathematic model,   cyclo(tyr-pro) was obtained 51.54 mg L-1 (model   expectations) and 50.04 mg L-1 from experiments. The   differences of experiment with expectation response value was   2.91%. Using the optimized parameters, the studies demonstrated   an increase in the cyclo(tyr-pro) was 2.5 fold from 20 mg   L-1 to 50 mg L-1.

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