rofiq sunaryanto - Isolation and Characterization of Antimicrobial Substance from Marine Streptomyces sp.

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  Journal of Microbiology Indonesia   Vol 4, No 2   (2010)


  Isolation and purification of antimicrobial active substance   produced by marine Actinomycetes has been carried out.   Marine sediment samples were obtained from six different places   at Banten West Coast. Isolation was conducted using two   pretreatment methods, acid and heat shock pre-treatment. A total   of 29 Actinomycetes isolates were obtained from the   various sediment samples collected, then tested for antimicrobial   activity against Escherichia coli ATCC 25922,   Staphylococcus aureus ATCC25923, Pseudomonas   aeruginosa ATCC27853, Bacillus subtilis ATCC 66923,   Candida albicans BIOMCC00122, and Aspergillus   niger BIOMCC00134. Among the isolates, isolate A11 was the   most activity to Gram-positive and Gram-negative bacteria, and   morphological observation and identification using 16S rRNA   showed that the isolate was similar to Streptomyces sp.   Production of active compound from A11 isolate used yeast peptone   medium. Purification of active compounds was carried out using   silica-gel-column chromatography and preparative HPLC. A single   peak of active compounds was detected by HPLC, which showed a   retention time of 8.35 min and maximum absorbance in UV visible   at 210 nm and 274.5 nm respectively.

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